Chemical synthesis of RNA including 5-taurinomethyluridine and 5-taurinomethyl-2-thiouridine
نویسندگان
چکیده
منابع مشابه
Chemical synthesis of novel taurine-containing uridine derivatives.
Recently, novel taurine-containing uridine derivatives were discovered in mammalian mitochondrial tRNAs, and these modified ribonucleosides existed at the first position of the anti-codon. This paper describes the chemical synthesis of these novel uridine derivatives, 5-taurinomethyluridine (tau m5U) and 5-taurinomethyl-2-thiouridine (tau m5s2U). These taurine-containing uridine derivatives wer...
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The synthesis of 5'-triphosphates of 4-thiouridine, 2-thiocytidine, 5-bromocytidine, and [6N]adenosine is described. All compounds are characterized by chromatographic and spectral data. Phosphorylation of corresponding nucleotides was performed using phosphoryl chloride and con densation with pyrophosphate. Intermediates were not isolated. The final products were purified by chromatography an...
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5-Taurinomethyluridine (τmU) and 5-taurinomethyl-2-thiouridine (τmsU) are located at the wobble position of human mitochondrial (hmt) tRNA and tRNA, respectively. Both hypermodified units restrict decoding of the third codon letter to A and G. Pathogenic mutations in the genes encoding hmt-tRNA and hmt-tRNA are responsible for the loss of the discussed modifications and, as a consequence, for t...
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A simple strategy is reported for 5'-adenylation of nearly any RNA sequence of indefinite length. The 5'-adenylated product (5'-AppRNA) is an activated RNA that is structurally similar to 5'-triphosphorylated RNA, which is usually prepared by in vitro transcription using T7 RNA polymerase. In the new 5'-adenylation strategy, the RNA substrate is first 5'-monophosphorylated either by T4 polynucl...
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A series of (5-nitrofuran-2-yl)-1, 3, 4-thiadiazole-2-yl derivatives 6a–6e have been synthesized and screened for in vitro anti-leishmanial activity against the promastigote form of L. major. The structure of Schiff bases were confirmed by 1H NMR, IR. Screening results indicate that all of the designed and synthesized final compounds (6a-6e) significantly reduced the viability of promastigotes ...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2008
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/nrn163